M.I.R.(A.S.)

6/17/02


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Table of Contents

M.I.R.(A.S.)

The only generally applicable way of solving macromolecular crystal structure

Problems

Stages

Techniques

Data collection

Scaling

Scaling

Comparison

Difference Pattersons

Difference Patterson

Non-isomorphism

Solving HA sub-structure

Shelxs input

Shelxs solution

Checking Solution

Refinement of solution

Cross/self phasing

Cross phasing of 2nd derivative

Refinement of sites

Refinement of all derivatives

Initial phasing

Initial phases

Initial (MIRAS) map

Density Modification

Solvent flattening

Solvent flattened map

NCS averaging

NCS averaged (phase extended) map

Phase Extension

Associated/Related methods

Example used

Author: JPD3

Email: a.w.ashton@dl.ac.uk

Home Page: http://www.ccp4.ac.uk