Slide 24 of 27
Running SCALA from ccp4i is very simple, as most parameters have been given sensible default values.
It is probably worthwhile altering two of the defaults:
- Change the scaling model from “On rotation axis” to “On rotation axis with secondary beam correction”; the surface plot provided with this latter option provides a useful check for strong absorption.
- Change the default rotation interval from 10 to 5 degrees, particularly if you have a large unit cell with plenty of data to scale.
It is worthwhile separating the anomalous pairs if performing a MAD experiment or similar.