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[ccp4bb] PhD and postdoctoral position at CBI / IGBMC, Strasbourg


Dear all,


we have 2 open positions for a PhD candidate and a post-doctoral researcher to join the Klaholz group at the Centre for Integrative Biology, IGBMC, Illkirch, France http://igbmc.fr/Klaholz


We are studying the structure and function of protein complexes through integrated structural biology and notably by high resolution cryo-EM.

We are looking for 2 highly enthusiastic and motivated scientists with relevant expertise in structural biology of DNA/RNA-protein complexes, ideally with good experience in cryo electron microscopy. For further details please see below.

For those present at the 3D-EM Gordon conference please feel free to contact me directly.


With best regards,


Bruno Klaholz





Some recent publications:


- L. Andronov, J. Michalon, K. Ouararhni, I. Orlov, A. Hamiche, J.-L. Vonesch & B. P. Klaholz. 3DClusterViSu: 3D clustering analysis of super-resolution microscopy data by 3D Voronoi tessellations. Bioinformatics, 2018, Apr 4, in press. doi: http://dx.doi.org/10.1093/bioinformatics/bty200 and https://www.biorxiv.org/content/early/2017/06/07/146456.

- O. von Loeffelholz, G. Papai, R. Danev, A. G. Myasnikov, S. K. Natchiar, I. Hazemann & J.-F. Ménétret, & B. P. Klaholz. Volta phase plate data collection facilitates image processing and cryo-EM structure determination. J. Struct. Biol., 2018, 202, 191-199. doi: http://dx.doi.org/10.1016/j.jsb.2018.01.003.

- I. Orlov, R. Drillien, D. Spehner, M. Bergoin, A. Abd-Alla, B. P. Klaholz. Structural features of the Salivary gland hypertrophy virus of the tsetse fly revealed by cryo electron microscopy and tomography. Virology, 2018, 514, 165-169. http://dx.doi.org/10.1016/j.virol.2017.11.016.

- S. K. Natchiar, A. G. Myasnikov, H. Kratzat, I. Hazemann & B. P. Klaholz. Atomic model building and refinement into high-resolution cryo-EM maps. Protocol Exchange 2017 http://dx.doi.org/10.1038/protex.2017.122

- S. K. Natchiar, A. G. Myasnikov, H. Kratzat, I. Hazemann & B. P. Klaholz. Visualization of chemical modifications in the human 80S ribosome structure. Nature, 2017, 551, 472-477. doi: http://dx.doi.org/10.1038/nature24482. Epub 2017 Nov 15.

- O. von Loeffelholz, S. K. Natchiar, N. Djabeur, A. G. Myasnikov, H. Kratzat, J.-F. Ménétret, I. Hazemann & B. P. Klaholz. Focused classification and refinement in high-resolution cryo-EM structural analysis of ribosome complexes. Curr. Opin. Struct. Biol., 2017, 46, 140-148. doi: http://dx.doi.org/10.1016/j.sbi.2017.07.007.

- B. P. Klaholz. The ribosome holds the RNA Polymerase on Track in Bacteria. Trends Biochem Sci., 2017, 42, 686-689. doi: http://dx.doi.org/10.1016/j.tibs.2017.07.003.

- K. Mohideen-Abdul, K. Tazibt, M. Bourguet M, I. Hazemann, I. Lebars, M. Takacs, S. Cianférani, B. P. Klaholz, D. Moras & I. M. L. Billas. Importance of the Sequence-Directed DNA Shape for Specific Binding Site Recognition by the Estrogen-Related Receptor. Front. Endocrinol. 2017, 8, 140. doi: http://dx.doi.org/10.3389/fendo.2017.00140.

- I. Orlov, A. G. Myasnikov, L. Andronov, S. K. Natchiar, H. Khatter, B. Beinsteiner, J-F. Ménétret, I. Hazemann, K. Mohideen, K. Tazibt, R. Tabaroni, H. Kratzat, N. Djabeur, T. Bruxelles, F. Raivoniaina, L. di Pompeo, M. Torchy, I. Billas, A. Urzhumtsev & B. P. Klaholz. The integrative role of cryo electron microscopy in molecular and cellular structural biology. Biol Cell., 2017, 109, 81-93. doi: http://dx.doi.org/10.1111/boc.201600042.

-  A. G. Myasnikov, S. K. Natchiar, M. Nebout, I. Hazemann, V. Imbert, H. Khatter, J.-F. Peyron & B. P. Klaholz. Structure-function insights reveal the human ribosome as a cancer target for antibiotics. Nat Commun., 2016, 7, 12856. doi: http://dx.doi.org/10.1038/ncomms12856.

-  A. Simonetti, J.-F. Ménétret, F. Martin, A. G. Myasnikov, Q. Vicens, L. Prongidi-Fix, S. K. Natchiar, B. P. Klaholz & G. Eriani. Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation. Nat Commun., 2016, 7, 12622. doi: http://dx.doi.org/10.1038/ncomms12622.

-  L. Andronov, I. Orlov, Y. Lutz, J-L. Vonesch & B. P. Klaholz. ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy. Sci. Rep. 2016, 6, 24084. doi: http://dx.doi.org/10.1038/srep24084.

-  L. Andronov, Y. Lutz, J-L. Vonesch & B. P. Klaholz. SharpViSu: integrated analysis and segmentation of super-resolution microscopy data. Bioinformatics, 2016, 32, 2239-2241. doi: http://dx.doi.org/10.1093/bioinformatics/btw123.

-  B. P. Klaholz. Structure sorting of multiple macromolecular states in heterogeneous cryo-EM samples by 3D multivariate statistical analysis. Open Journal of Statistics, Special Issue on Multivariate Statistical Analysis, 2015, 5, 820-836. http://dx.doi.org/10.4236/ojs.2015.57081.

-  B. Beinsteiner, J. Michalon & B. P. Klaholz. IBiSS, a versatile and interactive tool for integrated sequence and 3D structure analysis of large macromolecular complexes. Bioinformatics, 2015, 31, 3339-3344. doi: http://dx.doi.org/10.1093/bioinformatics/btv347.

-  H. Khatter, A. G. Myasnikov, K. Natchiar & B. P. Klaholz. Structure of the human 80S ribosome. Nature, 2015, 520, 640-5. doi: 10.1038/nature14427. Epub 2015 Apr 22.

-  Z. A. Afonina, A. G. Myasnikov, V. A. Shirokov, B. P. Klaholz, A. S. Spirin. Conformation transitions of eukaryotic polyribosomes during multi-round translation. Nucleic Acids Res. 2015, 43, 618-28. doi: http://dx.doi.org/10.1093/nar/gku1270.

-  A. G. Myasnikov, Z. A. Afonina, J-F. Ménétret, V. A. Shirokov, A. S. Spirin & B. P. Klaholz. The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes. Nat Commun., 2014, 5, 5294. doi: http://dx.doi.org/10.1038/ncomms6294.

-  M. Maletta, I. Orlov, P. Roblin, Y. Beck, D. Moras, I. M. L. Billas & B. P. Klaholz. The palindromic DNA-bound USP/EcR nuclear receptor adopts an asymmetric organization with allosteric domain positioning. Nat Commun., 2014, 5, 4139. doi: http://dx.doi.org/10.1038/ncomms5139.


For ongoing projects and full publication list of the team see http://igbmc.fr/Klaholz


Our group is located at the Centre for Integrative Biology (CBI) at IGBMC, Illkirch/Strasbourg, France, which comprises cutting-edge cryo-EM facilities:

The CBI provides a leading-edge scientific and technological environment in integrated structural biology to address the structure and function of biological systems, notably on gene _expression_, from the atomic, molecular to the tissue scales. The CBI http://www.igbmc.fr/grandesstructures/cbi/, hosts the French and European Infrastructures for Integrated Structural Biology, FRISBI http://frisbi.eu/  and Instruct https://www.structuralbiology.eu/  which comprises advanced electron microscopy facilities equipped with cutting-edge instrumentation such as Titan Krios and Polara cryo electron microscopes, cryo Focused Ion Beam Scanning Electron Microscope (cryo-FIB/SEM) and super-resolution fluorescence microscopy http://frisbi.eu/centers/instruct-center-france-1-igbmc/cryo-electron-microscopy/. The Titan Krios microscope is equipped with Cs corrector, Falcon 3 camera, GIF energy filter, K2 camera and phase plate. In addition, the EM facility has a suite of associated equipments for sample preparation and dedicated computing resources for image processing and 3D reconstruction by single particle cryo-EM and cryo electron tomography (cryo-ET).


Applications should be sent via email to klaholz@igbmc.fr including CV, list of publications, names of 3 referees and motivation letter. The postdoctoral position remains open until filled.


For the PhD position there is the possibility for a fellowship through the doctoral school at the University of Strasbourg, 2 research topics are available:



Deadline is June 11th 2018. Please contact me in advance if you are interested.





Bruno P. Klaholz

Centre for Integrative Biology

Department of Integrated Structural Biology
Institute of Genetics and of Molecular and Cellular Biology
IGBMC - UMR 7104 - U 1258

1, rue Laurent Fries
BP 10142

Tel. from abroad: 0033.369.48.52.78
Tel. inside France:




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