Dear CCP4 Community
I´d like to request your help to spread the word about the position described below:
FtsZ, the prokaryotic homologue of tubulin, is the universal driver of bacterial
cell division and an attractive target for new antibiotics. Our group has been
using a combination of approaches (genetics, live cell microscopy and structural
biology) to study the mechanism of FtsZ polymerization and how it is regulated
by modulator proteins. More recently, we established the conditions for
applying NMR to FtsZ. This breakthrough should allow addressing several
questions that have not been accessible by X‐ray crystallography, such as: 1)
Determination of the allosteric mechanism of polymerization; 2) Mapping of the
interaction with modulators that do not co‐crystalize with FtsZ; 3) Screening and
mapping binding sites of candidate small molecule inhibitors.
We are seeking highly motivated candidates, capable or working independently
and interested in bridging basic science with antibiotic discovery. Previous
experience with protein NMR is a plus, but not mandatory. Crystallographers
and biochemists with good protein _expression_ and purification skills are
welcome to apply. Applicants should send their CV and a brief description of
their past research experience to Frederico Gueiros‐Filho (firstname.lastname@example.org
and should organize 2 recommendation letters to be sent directly to the same
University of São Paulo (USP) is the #1 university in Latin America and is
consistently ranked among the top 100 universities of the world
of‐sao‐paulo#ranking‐dataset/558261). The Department of Biochemistry is one
of the leading research centers in Brazil, with an internationally recognized
faculty and world class facilities, including an 800 MHz NMR spectrometer. This
project involves collaboration with the group of Dr. Jose Manuel Andreu, CIB,
Madrid, and may include visits to his group.
Bisson‐Filho et al., (2015). PNAS 112:E2130‐E2138.
Blasios et al., (2013). PLoS One, 8:e60690.
Castellen et al., (2015). Biomolecular NMR Assignments 9:1‐5.
Nogueira et al., (2015). Biomolecular NMR Assignments 9:387‐91.