Contract:
Contract/Project
Salary
range: ≥ 35,000 and < 45,000€ annual gross
Employer:
Institut de Biologie Structurale de Grenoble
Workplace:
Grenoble - FRANCE
Skill
area: Biology, Medicine, Health - Physics
Application
deadline: 4/15/2011
The
Institut de Biologie Structurale (IBS), in Grenoble (France) is
a research
centre in a field that is essential for understanding
fundamental biological
mechanisms : STRUCTURAL BIOLOGY. IBS is also part of the
Partnership for
Structural Biology (PSB) whose primary objective is to study
proteins of
biomedical interest. The PSB constitutes a further step in the
development of
the region as an International centre of excellence for
structural biology.
The Partnership for Structural Biology include three
pan-European institutes:
the ESRF, the world’s foremost synchrotron X-ray source, the
ILL, a world
centre for neutron scattering, the EMBL, the Grenoble Outstation
of the
European Molecular Biology Laboratory.
Mission:
A
two-year post-doctoral position is asked in an application for a
grant funded
by « Vaincre la Mucoviscidose » and the CV of a candidate must
be added to the
project.
The candidate will be integrated in the « Heavy Metal and
Signaling » team at
the Institut de Biologie Structurale (IBS) de Grenoble
(http://www.ibs.fr/groups/metalloproteins-group/heavy-metal-and-signaling/),
close
to ESRF, and will participate to a project that aims at
addressing the
mechanisms by which the P. aeruginosa FUR protein interacts with
the first- and
second-generation peptide inhibitors already identified by our
partners. The
proposed study will complete the picture of a fundamental
physiological process
that is relevant to virulence (iron metabolism) and assess its
potential as a
new target (iron regulation) for alternative therapeutics. We
now need to
improve our knowledge on the FUR-antiFUR inhibitor interactions
and to obtain a
third generation of inhibitors. Deciphering the
structure-function
relationships of protein/inhibitor complexes will help to design
new
antibacterial molecules as well as to get insights into the FUR
protein
mechanism.
The candidate will focus on experiments related to biophysics of
protein-inhibitor interactions such as determination of the
thermodynamic
parameters (Biacore technology available in the IBS) and
determination of
structures by X-Ray diffraction. The HMS group will provide the
biochemistry
support of the project and our collaborators will bring their
experience in
conception of FUR inhibitors as well as interaction studies by
two-hybrid
and/or molecular docking.
Candidates profile:
Structural
biology, crystallization, X-ray diffraction, structure
determination,
thermodynamics, SPR (Biacore). Candidates must have obtained
their PhD since less
than two years.
Contact:
Send CV
and two letters of reference to:
jacques.coves@ibs.fr