Research Assistant – Membrane Protein Production/Crystallization
Membrane Structural and Functional Biology – Caffrey Lab
Trinity College Dublin, Ireland
The Membrane Structural and Functional Biology group seeks to solve the crystal structure of medically important membrane proteins for use in structure/function studies and for structure-based therapeutics development. Target proteins are involved in diabetes, obesity, cancer and pain and in communicable and other non-communicable diseases. In support of this activity, we seek a highly self-motivated, skilled and committed Research Assistant.
The Research Assistant will have primary responsibility for routine, repetitive and proven/robust molecular cloning, insect and mammalian cell maintenance, cell-based biomass production, protein purification and biochemical/biophysical characterization of membrane proteins, and for manual and robotic crystallisation extending from plate and screen preparation, to setup and evaluation, and on to archiving trial data. The Research Assistant will share responsibility for preparing common buffers stocks (for SDS-PAGE, DNA gels, protein purification and crystallization, etc.), LB liquid and solid medium/plates, cell-culture and molecular cloning, membrane washing (for protein purification), and regenerating resins. The Research Assistant will help with maintaining lab instruments, databases and inventories and with administration.
Knowledge & Experience
Training and support will be provided by in-house staff in all of the specialty methods and protocols used.
Salary €30,200 per annum
Closing Date September 14, 2016
Principal Investigator Prof. Martin Caffrey - email@example.com
Candidates are asked to submit a letter detailing education, training and laboratory experience. In the letter the candidate should outline why they feel they are suitable for the position in an interdisciplinary group that will involve extensive interaction with collaborators around the globe. The letter of application must be accompanied by a full CV to include the names and contact details of two referees (email addresses and phone numbers to be provided). At least one referee should be an individual the candidate has worked with and who has agreed to provide a letter of reference on the candidate’s behalf.
Applications should be emailed to: Shuang Leng LENGS@tcd.ie